Spectrophotometer detector malfunction
Detector malfunction or aging can significantly reduce sensitivity, causing peak intensities to drop below detection thresholds. Inconsistent sample preparation, such as variations in concentration or lack of homogeneity, can also lead to insufficient analyte levels for reliable. This guide is designed to help you identify and resolve the most common problems quickly and easily, ensuring your measurements. Tems points out three common sources of errors: To avoid sampling errors, a sample must not be so concentrated that it's absorbance is too high, and the sample must be placed accurately in the instrument so that light is passing. If you've ever wondered why absorbance readings seem inconsistent or why your baselines shift unexpectedly, you're not alone, and you're in the right place.
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